A METHOD OF STAINING BACTERIAL FLAGELLA
نویسندگان
چکیده
منابع مشابه
Staining bacterial flagella easily.
A wet-mount technique for staining bacterial flagella is highly successful when a stable stain and regular slides and cover slips are used. Although not producing a permanent mount, the technique is simple for routine use when the number and arrangement of flagella are critical in identifying species of motile bacteria.
متن کاملA simple, rapid method for demonstrating bacterial flagella.
We developed a simple, rapid method for demonstrating flagellation of bacteria using the fluorescent protein stain NanoOrange (Molecular Probes, Eugene, Oreg.). The NanoOrange reagent binds to hydrophobic regions of proteins, which results in substantial enhancement of fluorescence. Unbound reagent is essentially nonfluorescent. NanoOrange fluorescently stained bacterial cell bodies, as well as...
متن کاملFluorescent Staining Method for Bacterial Endospores
We have developed a quick, simple technique for the fluorescent staining of bacterial endospores. This technique was developed using Bacillus subtilis endospores. Our technique requires minimal sample heating so it can safely be carried out entirely in a glove-box. The endospores stain diagnostically as an oval with a standard fluorescent nucleotide stain, acridine orange.
متن کاملSynthesis of Bacterial Flagella
was developed. This procedure involved measuring the binding of purified, radioactively labeled, antiflagellar antibodies to bacteria. The assay was used to determine the requirements for ribonucleic acid (RNA) and protein synthesis during flagellar regeneration in Bacillus subtilis. Immediate inhibition of flagella development was observed when chloramphenical or puromycin was added to cells. ...
متن کاملBacterial Flagella: Flagellar Motor
Many species of bacteria actively seek out favourable conditions for growth by swimming up gradients of nutrients, oxygen, light or other attractants, or down gradients away from toxic substances (repellants). Different species employ several different modes of swimming, almost all of which are driven by the rapid rotation of helical flagellar filaments that protrude from the cell. Rotation of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1926
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.12.4.273-274.1926